GSM7430902: Barcode dataset of sample 2; Mustela putorius furo; RNA-S... - SRA

SRX20525318: GSM7430902: Barcode dataset of sample 2; Mustela putorius furo; RNA-Seq
2 ILLUMINA (Illumina NovaSeq 6000) runs: 75.9M spots, 10.2G bases, 3Gb downloads

External Id: GSM7430902_r1

Submitted by: Mayerlab, Max Planck Institute of Neurobiology.

Study: Barcode lineage tracking of ferret cortical progeny using TrackerSeq (Mayer lab)

PRJNA976704 • SRP439808 • All experiments • All runs

show Abstracthide Abstract

Diversity of cortical radial glia cells (RGCs) and their complex relationships to generate neurons in species with expanded germinal zones and a folded cortex, remains unclear. We used TrackerSeq, a technique that integrates DNA barcodes into the genome of electroporated RGCs, to identify the distinct cell lineages that shape ferret cortex. Overall design: E34 ferret embryos were electroporated with a piggyBac transposon-based library and eGFP+ cells were isolated at E37 by FACS and procesed for their parallel transcriptomic and barcode-lineage tracing characterization.

Sample: Barcode dataset of sample 2

SAMN35439749 • SRS17834762 • All experiments • All runs

Organism: Mustela putorius furo

Library:

Name: GSM7430902

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC SINGLE CELL

Selection: cDNA

Layout: PAIRED

Construction protocol: Embryonic tissue was collected in ice-cold Leibowitz medium and papain dissociation was carried out (Wortington, #LK003150). GFP-possitive cells were FACS sorted and after processed on the 10x Genomic Platform. Gel Beads-in-emulsion and cDNA generation carrying cell- and transcript-specific barcode was done using the Chromium Single Cell 3' Reagent Kit v3.1 (PN-1000121) following the manufacturer's protocol. Transcriptomic libraries were prepared for sequencing using standard Illumina protocols. Barcode libraries were prepared from PCR cDNA to amplify barcode regions with custumed primers with standard Illumina adapters.

Runs: 2 runs, 75.9M spots, 10.2G bases, 3Gb

Run	# of Spots	# of Bases	Size	Published
SRR24748650	37,794,282	5.1G	1.5Gb	2024-03-27
SRR24748651	38,144,016	5.1G	1.5Gb	2024-03-27

ID:: 27949027

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